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Widal Test (Tube Method) (SR0028)

Description

The Widal Test (Tube Method) is a serological test used to detect the presence of antibodies against Salmonella typhi and Salmonella paratyphi in the blood, aiding in the diagnosis of enteric fever (typhoid and paratyphoid fever). The test measures agglutinating antibodies (O and H) in the patient’s serum, which helps clinicians confirm typhoid fever or paratyphoid infection. This method is widely used in endemic areas to detect recent or ongoing infection. The tube method involves serial dilution of patient serum with standardized antigen suspensions to observe agglutination patterns, which are interpreted for diagnostic titers. The test is valuable in combination with clinical evaluation, and repeated testing may be necessary to confirm rising antibody titers. Proper blood collection, serum separation, and storage are essential for accurate results.

Report Time

Fasting

Not Required

Reporting Time

Same Day

Gender

Male/Female

Age Group

5-99 yrs.

Pre-Test Instructions:

  • Collect serum in a plain tube and allow clotting
    Separate serum promptly to avoid hemolysis
    Avoid hemolyzed or lipemic samples
    Provide patient clinical history if possible
    Repeat test may be needed after 7–10 days for rising titer confirmation
    Interpretation should consider local baseline antibody titers in endemic areas
    Cross-reactivity with other Salmonella species or infections may occur

Parameters Covered

रु 500

✔ Home collection available ✔ No hidden charges

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